1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride

��发布时间：2025-02-27 ��浏览次数：614次

 Chemical Name: 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride

CAS Number: 25952-53-8

Molecular Formula: C₉H₂₁ClN₂

Molecular Weight: 203.7 g/mol

Appearance: White to pale yellow crystalline powder

Solubility: Soluble in water, alcohol, and ether

Storage Conditions: Store in a cool, dry place, avoiding high temperatures and humidity.

What is 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride (EDC.HCl)?

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride (EDC.HCl) is a commonly used reagent in biochemistry and organic synthesis. It primarily functions as a coupling agent, facilitating chemical reactions between different molecules, particularly in peptide synthesis, protein modification, and polypeptide formation. EDC.HCl is highly effective in catalyzing the formation of amide bonds, making it an essential reagent in various synthesis processes.

Functions and Applications of EDC.HCl

Peptide Synthesis and Protein Modification

EDC.HCl is widely used in peptide synthesis, particularly in assembling amino acid sequences. It promotes the formation of amide bonds between amino acids or peptide chains by generating coupling intermediates, making it a crucial reagent in polypeptide synthesis.

Crosslinking Reactions

In biochemical research, EDC.HCl is used as a crosslinking agent. It enables the conjugation of amine-containing molecules with carboxyl-containing molecules, forming crosslinked structures. This application is valuable in protein structural studies, antibody production, and drug delivery system development.

DNA/RNA Conjugation

EDC.HCl is also employed in DNA and RNA conjugation reactions, particularly in genomics and molecular biology. It is used for synthesizing nucleic acid probes, drug carriers, and biological markers.

Surface Modification and Biosensor Applications

In biosensor development, EDC.HCl is commonly used for surface modification, facilitating the immobilization of biomolecules such as antibodies, proteins, and nucleic acids. This improves the sensitivity and specificity of biosensors.

Development of Peptide-Based Drugs

Due to its effectiveness in promoting peptide chain formation, EDC.HCl is widely used in developing peptide-based drugs. Peptide drugs, as bioactive molecules, are used in treating various diseases, including cancer, diabetes, and immune-related disorders.

Safety and Side Effects

EDC.HCl should be handled with care as it is a potent chemical reagent that may cause skin and eye irritation. Inhalation or excessive ingestion may negatively affect the respiratory and gastrointestinal systems. Proper protective equipment, such as gloves and safety goggles, should be worn, and it should be used in a well-ventilated environment.

Conclusion

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride (EDC.HCl) is a widely used reagent in biochemistry and organic chemistry, primarily for peptide synthesis, protein modification, and biomolecular conjugation reactions. Its high catalytic efficiency makes it an essential tool in many experimental and industrial applications. Proper safety precautions should be followed to prevent adverse health effects.