Guanidine Thiocyanate

��发布时间：2025-03-05 ��浏览次数：763次

 Chemical Name: Guanidine Thiocyanate

CAS Number: 593-84-0

Molecular Formula: CH5N3S

Molecular Weight: 118.14 g/mol

Appearance: White crystalline powder

Solubility: Easily soluble in water

Storage Conditions: Store in a cool, dry place, avoiding moisture and high temperatures.

What is Guanidine Thiocyanate?

Guanidine Thiocyanate is an inorganic chemical reagent widely used in biochemical experiments, particularly in RNA extraction, protein stabilization, and cell lysis processes. It effectively disrupts cell membranes and removes proteins, making it commonly used in RNA and DNA extraction procedures.

Functions and Applications of Guanidine Thiocyanate

1. RNA Extraction

Guanidine Thiocyanate is commonly used in molecular biology experiments, especially for RNA extraction. It disrupts cell membranes and removes proteins, enabling efficient extraction of pure RNA. It also prevents RNA degradation and helps maintain RNA integrity.

2. Protein Stabilization

In some biochemical experiments, Guanidine Thiocyanate is used as a stabilizer for proteins. It helps maintain the activity and stability of proteins by altering their conformation, particularly during long-term storage or experimental procedures.

3. Cell Lysis and Separation

Guanidine Thiocyanate is widely used in cell lysis, especially in RNA and DNA extraction processes. Its high salt content quickly breaks down cell membranes, releasing nucleic acids from within the cells.

4. Molecular Biology Research

In addition to RNA and DNA extraction, Guanidine Thiocyanate is also used in nucleic acid purification and protein denaturation experiments. Its powerful lysis ability makes it an indispensable reagent in molecular biology research.

5. Clinical and Pharmaceutical Research

In some clinical studies, Guanidine Thiocyanate is used to research gene expression in cells, particularly in studies related to RNA stability and gene expression analysis.

Safety and Side Effects

Guanidine Thiocyanate is toxic, particularly to the eyes, skin, and respiratory system. It may cause irritation or burns upon contact, so protective goggles, gloves, and masks should be worn when handling it. In case of accidental ingestion or skin contact, it should be immediately washed off, and medical attention should be sought. It should be stored away from heat sources and kept in a sealed environment.

Summary

Guanidine Thiocyanate is an important molecular biology reagent widely used in RNA extraction, protein stabilization, and cell lysis. Its powerful cell-disrupting properties make it a key tool in biochemical research and nucleic acid purification. However, due to its toxicity, it is important to follow strict safety protocols when using it.